ABSTRACT
The purpose of this chapter is to address issues of the applications of solution protein chemistry to proteomics that are not discussed in detail by others and to avoid, when possible, redundance in the coverage of information that is discussed in considerable detail in other sources.1-7
Traditional solution protein chemistry,8-12 including spectroscopy,13,14 ultracentrifugation,15-17 and chemical modication, is the basis for proteomics. The chemical modication of proteins has extensive use in proteomics in several different categories. Chemical proteomics could be described as the application of “classical” solution chemistry, including the use of alkylating agents such as isotope-coded afnity tags and uorescent probes in quantitative proteomics.18-25
Chemiproteomics, which on the surface would appear to be a closely related concept to proteomics, has been dened as an approach using small molecules as afnity materials for the discovery of small-molecule binding proteins.26 This approach is best characterized by the use of afnity chromatography.27-30 There has been another publication31 on chemiproteomics suggesting that it has not gathered traction as a descriptor.
