Integration of Diverse Data, Algorithms, and Domains
High-throughput (HTP) methods are providing a comprehensive description of cells at several levels (see Figure 6.1). HTP sequencing has deciphered the genomes of multiple species and DNA microarrays have provided extensive information about several transcriptomes. More recently, methods have been developed to analyze the proteome and interactome. In yeast, several HTP methods have been used to determine the expression levels [196, 500] and subcellular localization of proteins [256, 325]. The interactome of yeast is being determined by HTP methods such as yeast 2-hybrid [261, 526], tandem aﬃnity precipitation (TAP) , and HTP mass spectrometric protein complex identiﬁcation (HMS-PCI) .