ABSTRACT
Contents 6.1 General Introduction ....................................................................................................... 92 6.2 Th e Calpain System.......................................................................................................... 93
6.2.1 Introduction ......................................................................................................... 93 6.2.2 Analytical Methods .............................................................................................. 94
6.2.2.1 Chromatography and Calpain/Calpastatin Assays ................................. 94 6.2.2.2 Zymography ........................................................................................... 96
6.2.3 Concluding Remarks ............................................................................................ 97 6.3 Cathepsins ........................................................................................................................ 97
6.3.1 Introduction ......................................................................................................... 97 6.3.2 Analytical Methods .............................................................................................. 99
6.3.2.1 Extraction and Fractionation ................................................................. 99 6.3.2.2 Removal and Determination
of Cystatin Activity ................................................................................ 99 6.3.2.3 Activity Measurements ........................................................................ 100
6.3.3 Concluding Remarks .......................................................................................... 100 6.4 Proteasome (Multicatalytic Proteinase Complex) ............................................................101
