ABSTRACT
HILIC is used primarily for the separation of very polar compounds that are poorly retained by reversed-phase liquid chromatography (RPLC).1-23 Alpert24 described HILIC as a variant of normal-phase liquid chromatography (NPLC), where the stationary phase is more polar than the mobile phase, and analytes typically elute in an
13.1 Overview of HILIC/UPLC ........................................................................... 291 13.1.1 HILIC and HILIC Stationary Phases ............................................... 291 13.1.2 Overview of UPLC and HILIC-UPLC Applications ....................... 293
13.2 Case Study: Fast In-Process Analysis of Ioversol by HILIC-UPLC ............ 295 13.2.1 Introduction ...................................................................................... 295 13.2.2 Experimental ....................................................................................296 13.2.3 Results and Discussion .....................................................................297
