ABSTRACT
Glycosylation is one of the most common posttranslational modi“cations of proteinsit is estimated that over 50% of human proteins are glycosylated.1 Glycoproteins have great importance in biological systems with the glycan moiety acting as a key to the correct folding and action of the protein.2 The characterization of glycoproteins is important in the diagnosis and treatment of human diseases. For biopharmaceutical industry, consistent generation of the same glycoforms in different batches of recombinant protein is essential to retain the drug activity; thus glycoprotein analysis plays an important role in the production process. In addition, characterization of glycoproteins from patients with a speci“c disease can lead to the discovery of new biomarkers or targets for drug development.
