ABSTRACT
To purify specific cell types in sufficient quantity for clinical application, we developed methods to covalently immobilize proteins onto polystyrene surfaces. Further, we developed procedures to terminally sterilize the proteincoated polystyrene surfaces to provide a sterile platform for cell manipulation. Taking advantage of the multiple molded and extruded forms of polystyrene, we have produced devices known as RPR CELLectors® that can be used to manipulate up to 3 x 1010 peripheral blood mononuclear cells (PBMC). Using covalently attached mAbs or lectins, these devices can be used to specifically deplete or positively select cells bearing the target antigen (Ag). Cells captured by these devices can be cultured, activated, and genetically modified while still immobilized on the modified surface. This chapter describes several specific cell manipulations that can be performed on these surfaces. Moreover, new embodiments of this technology that now permit the automation of these cell-processing schemes are outlined. Finally, the use of these cell separation devices in several clinical trials in the areas of bone marrow transplantation, solid tumor therapies, and acquired immunodeficiency syndrome (AIDS) treatments are discussed.
