ABSTRACT
Adrian P. Gee University of South Carolina School of Medicine, Columbia, South Carolina, and M. D. Anderson Cancer Center, Houston, Texas
I. INTRODUCTION Selective cell separation is an essential component of many processes in biology and medicine. In the majority of cases, the aim is to achieve complete removal of the target population from a cell mixture, however, additional considerations can have a major impact on the method that is chosen to effect the separation. For some applications, a secondary aim may be to recover the selected cells for study for additional manipulation. In other cases, the purity of the separation may take precedence over the yield of selected cells, or vice versa. Factors such as processing time, viability of residual cells, and exposure to nonmedically ap proved reagents also must be considered when selecting the separation technique.
