ABSTRACT

For small-scale separations, the collection of rosetted cells and beads is easily accomplished by holding a permanent magnet to the outside of the mixing tube. The beads are drawn to the magnets within a few seconds, and after 2-3 minutes the nonrosetted cells may be aspirated or decanted. Magnetic holders are commercially available for 0.5-to 50-mL tubes (Dynal) (17), and for multiple separations, side-pull and bottom-pull magnetic racks are available for 12 x 75 mm tubes (Coming).