ABSTRACT
Abstract ................................................................................................. 359 18.1 Introduction ................................................................................ 360 18.2 Experimental Part ....................................................................... 361 18.3 Results and Discussion .............................................................. 364 18.4 Conclusions ................................................................................ 371 Acknowledgment .................................................................................. 372 Keywords .............................................................................................. 372 References ............................................................................................. 372
ABSTRACT
An assessment of the total antioxidant activity (AOA) of blood serum of patients with the liver disease by two chemiluminescent methods having different models of free radical oxidation: “Hb-H2O2-luminol” and
“ABAP-luminol” is carried out. The comparative analysis showed not high correlation of results (r = 0.798), that is explained, mainly, by a distinction of free radical initiation mechanisms and influence of some blood serum components (proteins and bilirubins) on initiation process. More strongly it is shown in model with “Hb-H2O2”. In this regard, more preferable in clinical practice for an AOA assessment it is necessary to consider model “ABAPluminol.” A comparison of antioxidant parameters of blood serum of patients with the affected liver with some general clinical blood characteristics, such as the content of uric acid, total and direct bilirubin, albumin, parameters of lipid metabolism is carried out. The aim and scope of this study is the comparative analysis of the total blood serum AOA of patients with liver pathology at parallel measurements by two chemiluminescence devices with various models of free radical oxidation for the aim of more suitable model choice in clinical practice. Comparison of antioxidant and some general clinical parameters of blood serum for patients with liver pathology are carried out to establish a correlation between these characteristics.
