ABSTRACT

All of the biochemical reactions discussed in the previous chapters involve a change in molar enthalpy (AH). For example, the reaction with glucose catalyzed by glucose oxidase [Chapter 4, Equation (91)] releases 80 kJ/mol. The change in temperature due to chemical reactions that take place for the different biosensor designs discussed so far has been assumed to be negligible. Isothermal conditions are generally assumed when interpreting the biosensor signal. Either the thermal mass of the biosensor is so large that heat is rapidly dissipated, or the temperature of the entire measuring device is regulated with a circulating water bath or an electrically heated metal block.