ABSTRACT
Abstract ................................................................................................. 171 3.1 Introduction .................................................................................. 171 3.2 Materials and Methods ................................................................. 175
3.2.1 Collection of Plant Materials ........................................... 175 3.2.2 Chemicals ......................................................................... 175 3.2.3 Successive Solvent Extraction ......................................... 175 3.2.4 Determination of Total Phenolics and Tannins ................ 175 3.2.5 Estimation of Total Flavonoids ........................................ 176 3.2.6 In vitro Antioxidant Studies ............................................. 177
3.3 Discussion and Results ................................................................ 179 3.3.1 Total Phenolics, Tannins, and Flavonoids. ...................... 179 3.3.2 Radical Scavenging Activity Using DPPH Method ....... 180 3.3.3 TAA by 2, 2'-Azinobis (3-Ethylebenzothiozoline-6-
Sulfonic acid) (ABTS+) Assay .......................................... 181 3.3.4 Ferric Reducing Antioxidant Power (FRAP) Assay ........ 181 3.3.5 Metal Chelating Activity .................................................. 181
3.3.6 Phosphomolybdenum Assay ............................................ 182 3.4 Conclusion ................................................................................... 183 Keywords .............................................................................................. 183 Acknowledgment .................................................................................. 184 References ............................................................................................ 184
ABSTRACT
India is rich in biodiversity, ethnicity, geography, languages, arts, and culture. Indian traditional system of medicines like Ayurveda, Siddha, and ethnomedicine offer much useful, effective and simple herbal medicine. Glycosmis pentaphylla, of the family rubiaceae is used against cough, rheumatism, anemia and jaundice. The leaves of this plant are used in the treatment of fever, skin, and liver complaints. Glycosine, arborine, arborinine, glycerine, and glycosamine are some of the major alkaloids found in leaves. Glycosmis pentaphylla leaf extract was obtained by boiling the leaves in distilled water for 24 hr. The extract was dried and estimated for its phenolic, tannin, and flavonoid contents. Further, antioxidant property was measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azino-bis (ABTS+), metal chelating, ferric reducing ability of plasma (FRAP), and phosphomolybdeum assays. The total phenolic, tannin, and flavonoid content were found in moderate amount. All the assays proved to have reasonable radical scavenging activity when compared to standards. Hence, further research is going on in the laboratory to figure out the mechanism of action and compounds responsible for having antioxidant property by molecular and analytical tools.
