Rapid Isolation of Speci„c cDNAs or Genes by PCR

A full-length cDNA can be ampli„ed via RT-PCR. To achieve this objective, a forward or sense primer and a reverse or antisense primer should be designed in the

5′-UTR (untranslation region) and the 3′-UTR regions of a known cDNA sequence or based on a known amino acid sequence.