ABSTRACT
DNA and RNA sequencing is an essential technique mandated for DNA cloning, characterization, mutagenesis, DNA recombination, and gene expression.1-3 Very recently, nucleic acid sequencing has become a rapid and powerful tool to identify novel gene(s) targeted by a novel drug or protein. To „nd out new target molecules, one may not need to use traditional gene or cDNA cloning methodologies; nor is the isolated gene or cDNA needed to be completely sequenced. Instead, as long as a portion of exon sequences is sequenced, a potentially novel DNA molecule can be identi„ed by computer database searching. Nevertheless, nucleic acid sequencing is a crucial tool for one to ful„ll such a task. How does one sequence the DNA of interest? How does one identify the existence of mRNA expression in a cell-or tissue-type of interest? Which methodology is the best selection for your purposes? This chapter summarizes the author’s extensive experience and will help you achieve your goals.
