ABSTRACT
Since the Nobel Prize laureate Richard Feynman in 1959 proposed the idea about manipulating the material structure at the atomic level, nanoscience and nanotechnology have been developed as special areas of research involving a broad spectrum of methodologies from physics, chemistry, and biology. In Feynman’s historical lecture called “There’s Plenty of Room at the Bottom,” he postulated several principally new technologic ideas in elds such as information
processing and storage, mechanical construction of small devices, friction and lubrication problems, and microsurgery [1]. Notably, he pointed also on the importance of collaboration between scientists of different scientic areas. Talking about the progress in biology, Feynman indicated on several molecular problems that the biologists solved (or still continue to study!) only in next decades, for example, how proteins are synthesized or how the light is converted into chemical energy in the nature. He stressed also the important role of
6.1 Introduction ....................................................................................................................................................................... 93 6.2 Native Viruses as Nanoparticle Components .................................................................................................................... 94 6.3 Creating Articial Viral Nanoparticles ............................................................................................................................. 96
6.3.1 Construction of New Virus-Like Particles ............................................................................................................ 96 6.3.1.1 Basic Research Data ............................................................................................................................... 96 6.3.1.2 Virus RNA/DNA Sources ...................................................................................................................... 96 6.3.1.3 Infectious cDNAs/Cloned Structural Genes ........................................................................................... 96 6.3.1.4 Expression Vector/Host Systems ............................................................................................................ 97
6.3.2 Purication of Viral Nanoparticles ..................................................................................................................... 100 6.4 Modication of Viral Nanoparticles .................................................................................................................................101
