chapter
Mycological Media
Pages 34

Agar ..............................................................................................20.0g Glucose .........................................................................................20.0g Bi2(SO3)2.........................................................................................8.0g (NH4)2SO4.......................................................................................3.0g KH2PO4...........................................................................................3.0g MgSO4·7H2O ................................................................................0.25g CaCl2·2H2O...................................................................................0.25g Biotin ......................................................................................... 10.0g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121°C. Cool tubes in a slanted position.