chapter
Virus/Tissue Culture Media
Pages 12

Fetal bovine serum.................................................................... 10.0mL NaHCO3 (7.5% solution) ............................................................ 1.0mL Penicillin-streptomycin solution ................................................. 1.0mL Amphotericin B solution............................................................. 0.1mL

pH 7.2-7.4 at 25°C

Eagle MEM in Hanks BSS: Composition per liter: NaCl ................................................................................................8.0g Glucose ...........................................................................................1.0g KCl..................................................................................................0.4g CaCl2·2H2O...................................................................................0.14g MgSO4·7H2O ..................................................................................0.1g KH2PO4.........................................................................................0.06g Na2HPO4 .......................................................................................0.05g L-Isoleucine .................................................................................0.026g L-Leucine.....................................................................................0.026g L-Lysine.......................................................................................0.026g L-Threonine .................................................................................0.024g L-Valine .....................................................................................0.0235g L-Tyrosine....................................................................................0.018g L-Arginine .................................................................................0.0174g L-Phenylalanine.........................................................................0.0165g L-Cystine .....................................................................................0.012g L-Histidine....................................................................................8.0mg L-Methionine ................................................................................7.5mg Phenol Red...................................................................................5.0mg L-Tryptophan ................................................................................4.0mg Inositol .........................................................................................1.8mg Biotin ...........................................................................................1.0mg Folic acid......................................................................................1.0mg Calcium pantothenate ..................................................................1.0mg Choline chloride...........................................................................1.0mg Nicotinamide................................................................................1.0mg Pyridoxal·HCl ..............................................................................1.0mg Thiamine·HCl ..............................................................................1.0mg Riboflavin ....................................................................................0.1mg

Preparation of Eagle MEM in Hanks BSS: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.