ABSTRACT
The urgency for enhanced analytical methods for the evaluation of nutraceuticals is summarized well by Kathie Wrick in an excellent review about the impact of regulations in the book entitled Regulation of Functional Foods and Nutraceuticals “. . . a very real problem is the absence of validated analytical methods for use in manufacturing controls and nished product testing to assure that label claims, and therefore customer expectations, are met . . . Natural products are some of the most complex
Introduction ............................................................................................................ 157 Unique Strengths of NMR ................................................................................ 158 Automation in NMR ......................................................................................... 162 Seeing Beauty in a Highly Complex Mixture ................................................... 163
Targeted Approaches of Qualitative and Quantitative Assessment ........................ 163 Development of an NMR SBASE ..................................................................... 166 Obtaining Quantitative Results ......................................................................... 168
Nontargeted NMR Approaches of Qualitative Assessment ................................... 170 Sample Collection and Preparation Considerations for Botanical Metabolomics .................................................................................................... 170 NMR Acquisition and Processing for Metabolomics Analysis ......................... 172
Three Examples: NMR Analysis of Nutraceuticals and Botanicals ...................... 173 Ginseng ............................................................................................................. 173 Energy Drinks ................................................................................................... 173 Undeclared Drug Substance in Regenerect ....................................................... 177
Practical Considerations to Implementing NMR Screening of Nutraceuticals ..... 178 Conclusions ............................................................................................................ 178 References .............................................................................................................. 179
matrices found in the world of analytical chemistry. Sometimes they contain thousands of phytochemicals in one plant, which may vary in composition and quantity depending on season and soil . . . well designed clinical trials for many herbs and botanicals await standardized methods to validate plant species and analytical methods to establish a quantitative chemical ngerprint for the preparations under study” [1].
