ABSTRACT
ABSTRACT Cancer stem cells (CSCs) have been categorized in a large number of hematopoietic cancers and solid tumors and are identied by virtue of the expression of cell surface markers. These CSCs have been isolated from the bulk tumor by the expression of cell surface proteins, like CD44, CD24, and CD133, and efux of Hoechst dye or aldehyde dehydrogenase (ALDH) activity by ow cytometry and/or uorescence-activated cell sorting (FACS). The identication of markers allows the prospective isolation of CSCs from whole tumor tissues, which would lead to the elucidation of important biological properties of CSCs and provide the possibility to target them. This chapter reviews the advances in this eld of cancer stem cells in various cancer types.
