ABSTRACT

The formation of BAs is inuenced by a number of factors such as FAA content and availability; microorganisms capable of producing decarboxylases; the nature of the medium; processing; and storage conditions (Figure 35.1). All these factors are interdependent and act together in various combinations. The combined action of these factors mainly determines nal concentrations of BAs, because it either directly or indirectly affects the microbial growth and then determines the presence and the activity

35.1 Introduction 675 35.1.1 Importance of Biogenic Amine Determination 676 35.1.2 Legislation of Biogenic Amines in Food and Beverages 677

35.2 Analytical Methods for Biogenic Amine Determination 678 35.3 Determination of Biogenic Amines by Flow Injection Analysis 678

35.3.1 Extraction of Biogenic Amines 678 35.3.2 Separation, Identication, and Quantication of Biogenic Amines

by FIA 680 35.4 Conclusion 685 Acknowledgments 686 References 686

of substrate (FAA) and enzyme (Ruiz-Capillas and Jiménez-Colmenero, 2004, 2009a; Roig-Sagués et al., 2009).