ABSTRACT

Thin-layer chromatography (TLC) is probably one of the most frequently used and by far the simplest chromatographic techniques used to separate or purify compound mixtures. Commercially available TLC plates that are used in laboratories allow resolution of compounds on many types of stationary phases such as thin-layer silica, reversed phase (RP) modied silica, cellulose, or aluminum oxide. TLC technique is widely used because it has many important advantages such as low cost, low solvent consumption, simultaneous elution of many different samples on one TLC plate, and no “memory” effects [1-2].