ABSTRACT
In analysis of the primary cannabinoids (Δ9-tetrahydrocannabinol, cannabidiol, and cannabinol) in marijuana samples (Cannabis sativa L.), diverse eluent systems have been described in the literature for use in chromatographic runs, such as ethyl acetate:methanol:water:concentrated ammonium (12:5:0.5:1), toluene:chloroform (7:3), and n-hexane:diethyl ether (80:20) [1]. The detection of cannabinoids can be accomplished using diverse diazonium salts, such as Fast Blue B, or with DuquenoisLevine reagent (Table 16.1) [2]. The limit of detection (LOD) varies from 2 to 10 ng mL−1 (Figure 16.1).
