chapter  8
96 Pages

FACTORS AFFECTING SEPARATION BEHAVIOR

As emphasized previously, the elution mechanism of proteins in

ion -exchange chromatography (lEe) is very complicated, since

a number of factors are involved in the elution process. For

the prediction of operating conditions and lor the design of col-

umn dimensions, therefore, it is necessary to know how one

particular variable affects the elution (separation) behavior

while the others are fixed.