chapter
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PolystyrenebasedFlashPiatesmakeanexcellentplatformforradioimmunoassays (RJAs).WhenworkingtoconvertastandardoptimizedRIAtoaFlashPlateassaythe primaryantisera(foranassayestablishedasacompetitionofthetestsampleforthe radioactivelylabeledantigen)willneedtobecoatedontoaFlashPiate.Butthetiteris frequentlysimilartotheoneusedforthereferenceassay.Thesamplepreparationand buffersdonotusuallyneedadjustmenteither.Thecountingefficiencyforthevarious isotopesisreducedoverclassicalscintillationcountingasdescribedabovebutduetothe lowerbackground,thesignaltonoiseratioisnotsacrificed.Thusthereisnoneedto increasetheamountofradioactivityusedintheFlashPlateassaysoverwhatisusedin otherformats.