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. 2 5 . 2

InoursystemwelookforIC50srangingwellintothehundredsofmicromolar,anda typical"hitrate"againstoneofourproteinsisI0to20percent,ratherthanthemore common0.1toIpercentfrequencyofconventionaldiscoveryscreens.Wesoonrealized thatitwaseasiertotitrateallcompoundsfromtheoutsetratherthanscreenfirstand titrateonlyhits.