65 Pages

Drug Drug Pharmacological Response mRNA Response

The method we will be describing to quantify gene transcription is quantitative polymerase chain reaction (QPCR). Compounds discovered with this approach can interact with any molecular targets from cell membrane events (e.g., receptors or ion channels) to intracellular messengers to nuclear targets (e.g. transcription factors). We will be describing the development of a gene expression-based screening (GEBS) program using this "marker" approach .