chapter
9 Pages

ofP eople and Machines

Table 1. Timings for individual assay assembly processes Time required to process screens

comprising: Screening Unit operation Completion of 2,000 compounds 10,000 compounds function unit operation (26 microplates) (130 microplates) assembly 96-well plate to 36 seconds 16 minutes 78 minutes

plate transfer assembly add 50J.1l reagent 20 seconds 9 minutes 43 minutes

to microplate assembly vortex microplate 10 seconds 4 minutes 22 minutes assembly vacuum filtration 75 seconds 33 minutes 2.7 hours assembly microplate wash 1 minute 26 minutes 130 minutes

(4 cycles) detection read absorbance 15-20 seconds* 7-9 minutes 33-43 minutes detection read fluorescence 45-90 seconds* 20-39 minutes 98-195 minutes detection scintillation 10-20 minutes** 4.3-8.6 hours 21.7-43.3 hours

counting

The microplate throughput llmmgs represent rates that can be routinely attained during the screening process. Although different manufacturers equipment will perform assay assembly tasks at slightly different rates, the timings are representative for the available equipment types. * Depending on equipment; ** Depending on required counting time.