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ofE ncoded Small Molecule Libraries via ECLiPS

Each of the thirty-one resin samples 8-38 was then uniquely binary encoded with a distinct set of five molecular tags. Resin samples ·s - 38 were again pooled to give batch B, the Fmoc protecting groups removed, and the resin split a final time into thirty-one batches in preparation for the third combinatorial step. The 31 reagents used included functionalized sulfonyl chlorides, isocyanates, carboxylic acids and chloroformates, producing sulfonamides, ureas, amides and carbamate linkages respectively (Figure 11). Binary encoding of the resulting 31 resin batches 39 - 69 was completed with a further distinct set of five molecular tags. Final pooling of the resin to give batch C was followed by removal of any acid-labile protecting groups (Figure 12). The completed library of 6727 members represents all possible permutations of R 1-R2-R3 (Figure 13).