ABSTRACT
Some of the most recent and important findings in cell molecular biology have been obtained not only by means of biochemistry and molecular biology techniques, but also by staggering improvements in light microscopy.1•2 Developments in this field are many: implementation of laser beam and stage scanning systems incorporating confocal optics; new electro-optical detectors with great sensitivity, linearity, and dynamic range; the possibility of two-dimensional (2-D) fast image enhancement, reconstruction, analysis, and three-dimensional (3-D) display; and application of luminescence techniques that provide the possibility to investigate the chemical and molecular details of life processes, thanks to their sensitivity, specificity, and potentiality for yielding spatial information. 3·6
The purpose of digital image restoration is to provide images with a higher spatial resolving power with respect to the original degraded images, by increasing sharpness and clarity, thus enhancing fine structure.
