Toxin Formation Clostridia
When competent investigators disagree, an enigmatic situation exists. The old adage must apply, "When experienced researchers have different findings , the same experiment was not done."
Publications on toxigenicity of the Clostridium tetani L-phase were contradictory, but the discrepancy has been clarified. A first reportS indicated that the L-variant lacks pathogenicity, but subsequently Rubio-Huertos25 recorded that the wall deficient stage makes as much or more toxin than the parent. This report from Spain dealt with variants produced by large quantities of glycine in the culture medium. Was there an essential difference in toxigenicity between glycine-induced variants and those induced by penicillin?