ABSTRACT
Affinity-based analytical methods and mass spectrometry occupy important positions in bioanalytical laboratories. Both possess extensive capabilities to detect, identify, and quantitate targeted analytes, and it is safe to say that thousands of applications have been developed involving one or the other. Many examples demonstrate the ability of these techniques to perform highly specific and sensitive assays. Nevertheless, the accelerated development of new pharmaceutical entities and the requirement to determine pharmacokinetic and pharmacodynamic properties in biological matrices present important analytical challenges that reach beyond the limitations of each technique.
