ABSTRACT
DNA affinity chromatography is a technique used to purify DNA-binding proteins [110]. Usually, DNA coupled to a suitable solid matrix is used for this purpose. Elution of the retained proteins can later be achieved through one of many methods, including the use of a salt or heparin, oligonucleotide competition, ligand-specific competition, or a change in temperature. This chapter discusses the various types of DNA affinity chromatography and examines some of its applications.
