ABSTRACT
Evaporative light-scattering detection is technically a quasi-universal detection technique for LC, as high-volatility analytes are not detected; however, as most solutes are less volatile than the eluting solvents, in practical terms ELSD can be considered a universal LC detector [24]. ELSD has found particular application for detection of substances without chromophores, which are not sensitively detected by UV absorbance without cumbersome, error-prone derivatization. General classes of substances for which ELSD finds unique application include lipids [25,32], carbohydrates [33,34], hydrocarbons [33,35], surfactants [36], and polymers [29,37]. However, ELSD is also beginning to find more general application, to include use in areas such as detection of amino acids [38], pharmaceuticals [39], and combinatorial libraries [40]. Figure 9 is a typical chromatogram for a separation of lipids with detection by ELSD.
