ABSTRACT
Capillary zone electrophoresis (CZE) offers several advantages in comparison to other CE separation modes. In most cases, CZE is simple and straightforward to perform: a single buffer is used throughout the capillary and electrode vessels, and sample is introduced as a zone or plug at one end. Capillary preparation often involves only filling the capillary with the separation buffer, although uncoated capillaries generally require prior washing or conditioning steps. The technique is inexpensive in comparison to capillary gel electrophoresis or isoelectric focusing, since low-cost buffers and salts are used. Separation selectivity can be easily varied by manipulation of buffer pH or use of additives. For these reasons, CZE is the most widely used CE mode for protein separations.
