chapter  6
104 Pages

Capillary Isoelectric Focusing

The natural end of an analysis is when resolution of the sample components of interest has been achieved. In practice, however, most assays are terminated arbitrarily. The end point of the experiment is determined by the researcher, and it is usually based on factors that include time and space (e.g., before the proteins of interest exit the gel in slab gel electrophoresis, or after all analytes have eluted from a chromatographic column). But there is also a small group of techniques in which the end point is determined by the attainment of equilibrium. These techniques are collectively called isoperichoric, meaning "equal to its environment" [282].