ABSTRACT
Compared with circular dichroism (CD) investigations of proteins in solution, rather few CD studies have been made of proteins localized at interfaces. The majority of the systems studied consist of proteins (or peptides) either adsorbed to or incorporated into artificial biological membranes. Such systems can be considered as liquid-liquid interfaces with which proteins are in one way or another associated. The even more scarcely studied category comprises proteins adsorbed at microscopic solid interfaces that are dispersed in aqueous solution. With respect to both situations, the question arises of whether the protein retains or changes its conformation upon interaction with the interfaces. Answers can be obtained by using CD spectroscopy as illustrated in this chapter.
