ABSTRACT
The object of an LC analysis is to establish the probable identity, and determine the precise amount, of each of the pertinent components present in the sample. The pertinent components may include all the substances present in the mixture or only those of specific interest. The identity of the peak is determined from its position on the chromatogram, that is, the time required for it to be eluted, whereas, the quantity of a component present is determined from the peak height or peak area. It must be emphasized that a single LC analysis on a hitherto unknown sample can not unambiguously confirm the presence of a particular compound on the basis of retention data alone. Retention data, whether it is corrected retention volume, capacity factor or the separation ratio of the solute to that of a standard, can only indicate the probability of substance identity. Retention data from a second analysis, using a different phase system, increases the confidence level but absolute verification requires confirmation by another analytical technique. This might include infrared spectrometry, mass spectrometry or nuclear magnetic resonance spectroscopy. Such evidence would be essential for litigation purposes.
