ABSTRACT

Takeuchi et al. [1] described an indirect photometric detection method for anions in micro high performance liquid chromatography. Micro anion exchange octadecylsilica columns permanently coated with the hydrophobic cetyltrimethylammonium ion (cetrimide) were used in the high performance liquid chromatography of chloride, nitrate and sulphate contained in potable water. Indirect detection of analyte anions was achieved with an ultraviolet detector. The breakthrough volume of the anion-exchange column was measured with aqueous sodium salicylate (1mM). Retention times of analyte anions in an artificial mixture were longer when aqueous sodium salicylate (1mM) containing acetonitrile (5%) was used as the mobile phase.