Affinity Separation Of Enzymes Using Immobilized Metal Ions Pgma Grafted Cellophane Membranes: b- Galactosidase Enzyme Model

Immobilized Cu+2‏‏ions affinity poly (glycidyl methacrylate) (PGMA) grafted cellophane membranes have been prepared, characterized and examined in the separation process of β-galactosidase (β-Gal) enzyme from its mixture with bovine serum albumin (BSA). The article focus on the step of Cu+2‏ions immobilization onto sulfonated PGMA grafted membranes. Maximum amount of immobilized Cu+2 ions was found to be 60.9 ppm per g of polymer. The verification of the immobilization of Cu+2‏ions step has been performed through characterization of the obtained membranes using EDAX analysis. Finally, Cu+2‏immobilized membranes have been evaluated in separation of β-Gal enzyme from its mixture with BSA in different pH medium. Maximum protein adsorption, for both proteins, has been obtained at pH range 4-4.5; as 90 and 45% for β-Gal and BSA, respectively, from (1:20) mixture solution.