ABSTRACT
Determine Conditions for Adsorbent Use in HGMF . . . . . . . . . 99 3.3 Design and Set-Up of Magnetic Separator Systems . . . . . . . . . . . . . . . . . . 99 3.4 Parameters Affecting System Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
3.4.1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102 3.4.2 Simplified Yield Estimation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104 3.4.3 Multi-Component Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 3.4.4 Case Study III: Optimization of the Capacity Ratio Used. . . . 109 3.4.5 Process Productivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 3.4.6 Case Study IV: Influence of Washing and Elution Steps . . . . . 114 3.4.7 Adsorbent Reuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 3.4.8 Case Study V: Pilot Plant Efficiencies . . . . . . . . . . . . . . . . . . . . . . . . 117
SHUKLA: “dk3347_c003” — 2006/5/23 — 17:54 — page 84 — #2
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Magnetic adsorbents possess a very powerful and unique handle that permits their selective manipulation within and from most (if not all) kinds of biological feedstock, simply through the application of a magnetic field. This ease of manipulation stands in stark contrast to all other adsorbents, and has been exploited in bioprocessing to develop a first capture step for proteins and other species from crude feedstocks known as high-gradient magnetic fishing (HGMF) [1-14].
