ABSTRACT
Dižuse optical spectroscopy (DOS) employs model-based photon-migration methods to quantitatively separate light absorption spectra from scattering spectra in multiple scattering thick tissues. Dižuse optical spectroscopic imaging (DOSI) extends this technology to an imaging format in order to map spectra from discrete regions of tissue. Bandwidth is achieved by combining time-independent spectroscopy with frequency-domain photon migration (FDPM), reducing dependence on the availability of speci›c diode lasers while expanding spectral content to hundreds of near-infrared (NIR) wavelengths (∼600-1000 nm). ™is combined approach improves the accuracy of absorption and scattering spectra calculations and allows simultaneous characterization of absorption contributions from several endogenous and exogenous chromophores (Bevilacqua et al. 2000).
