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Color Inserts

Figure 5.9 Effect of myosin extraction on SHG from myofibrils. Panels a and b show myofibril SHG images before and after myosin extraction, respectively. Panels c and d show two-photon excitation images of actin labeled with rhodamine-phalloidin before and after myosin extraction, respectively. Panels e and f show a superposition of SHG image (in red) and TPE image (in green) before and after the extraction. The size of the images is 20 × 20 μm2. (Modified from Vanzi, F. et al. 2006. J Muscle Res Cell Motil 27, 469-479. With permission.)