ABSTRACT
This chapter aims to provide an understanding of the factors governing bioreactor design, operation, and applications with special emphasis on the following aspects:
• Unraveling and describing the scientic principles underpinning bioreactor design, bioreactor instrumentation, and control
• Describing bioreactor assembly and operation • Describing how a bioreactor can be adapted to a range of specic applications
15.8.6 Exit Gas Analysis .................................................................................................. 436 15.8.6.1 Infrared Carbon Dioxide Analyzer ....................................................... 437 15.8.6.2 Paramagnetic Oxygen Analyzer ........................................................... 437 15.8.6.3 Mass Spectrometer ............................................................................... 437
15.9 “Substrate Sensors” ............................................................................................................ 437 15.9.1 Glucose Measurement and Control ....................................................................... 438 15.9.2 Methanol Measurement and Control..................................................................... 438
15.10 Bioreactor Preparation and Use .......................................................................................... 438 15.10.1 Disassembly of the Vessel ..................................................................................... 438 15.10.2 Cleaning ................................................................................................................ 438 15.10.3 Preparations for Autoclaving ................................................................................ 438 15.10.4 Autoclaving ...........................................................................................................440 15.10.5 Setup after Autoclaving.........................................................................................440 15.10.6 Inoculation of a Bioreactor Vessel ........................................................................ 441 15.10.7 Sampling from a Bioreactor Vessel .......................................................................442
