ABSTRACT

Geometry-Based Design ........................................................................................ 345 Methodology ................................................................................................. 345 Examples ....................................................................................................... 347

Stereochemistry-Based Design ..............................................................................348 Methodology .................................................................................................348 Example: Integral Membrane Proteins ......................................................... 349 Example: Nucleic Acids ............................................................................... 351 Example: Peptides ........................................................................................ 352 Example: Proteins ......................................................................................... 353 Example: Small Molecules ........................................................................... 353

Catalysis ................................................................................................................. 354 Early Efforts .................................................................................................. 354 Advanced Enzyme Design: Combining Geometry-Based and

Stereochemistry-Based Design ......................................................... 355 Applications of Computationally Designed Proteins ............................................. 357

Biosensors ..................................................................................................... 357 Therapeutic Proteins and Antibodies ............................................................ 358

Cytokine Design ............................................................................... 358 Antibody Afnity Maturation ........................................................... 359 Surpassing the Technical Limitation of the Lab ............................... 359

Conclusions ............................................................................................................360 Looking Back ................................................................................................360 Looking Forward ..........................................................................................360

References .............................................................................................................. 361