ABSTRACT
Introduction ............................................................................................................ 132 Techniques for Analysis of Glucosamine .............................................................. 133
Radiolabeling .................................................................................................... 133 HPLC Separation .............................................................................................. 134 Precolumn Derivatization Agents for UV/Fluorescent Visualization .............. 134 Postcolumn Agents Used for Indirect Fluorescent Detection ........................... 136 HPLC Using Electrospray Ionization-MS Detection ........................................ 136 The Use of HPLC with Alternative Detection Methods ................................... 137 Gas Chromatography ........................................................................................ 138 High-Performance Thin-Layer Chromatography ............................................. 138 Capillary Electrophoresis Determination ......................................................... 138 Capillary Electrophoresis with Dansylation of Glucosamine under
Microwave Irradiation .................................................................................. 139 Microchip Capillary Electrophoresis with Fluorescamine Labeling for
Anomeric Composition Determination ........................................................ 139 The Pros and Cons of Capillary Electrophoresis .............................................. 140 Infrared Spectroscopy with Chemometrics ...................................................... 140
Techniques for Analysis of Coenzyme Q10 ............................................................ 141 HPLC ................................................................................................................ 142 UV or Electrochemical Detection? ................................................................... 142 Column Switching HPLC ................................................................................. 143 Mass Spectrometry Detection ........................................................................... 143 Derivative Spectrophotometry .......................................................................... 144
Voltammetric Determination ............................................................................ 145 Square-Wave Voltammetry with Glassy Carbon Electrode .............................. 145 Mercury Hanging Electrode ............................................................................. 145 Electron Paramagnetic Spectroelectrochemistry .............................................. 145
Conclusions ............................................................................................................ 146 References .............................................................................................................. 146
INTRODUCTION
Over the past two decades, the public has gained an increasing interest in the role of diet, nutrition, and dietary supplements in the prevention and treatment of disease [Childs and Poryzees 1998]. This has resulted in a dramatic increase in the use of commercially available nutraceuticals and their foodstuffs [G. Lockwood 2007].
