ABSTRACT
The fluorescence lifetime is defined as the average time the fluorophore spends in the excited state after absorption of a photon. Fluorescence lifetimes can be measured with various approaches that can be broadly divided in time-domain and frequency-domain methods (Bastiaens and Squire 1999). These methods and standard approaches to analyze data acquired with these instruments are described in the various chapters of this book. In this chapter, we describe the global analysis methods developed for frequency-domain data (Clayton, Hanley, and Verveer 2003; Verveer, Squire, et al. 2000; Verveer et al. 2001a, 2001b; Verveer and Bastiaens 2003).
