ABSTRACT

Single-frequency CARS microscopy (Duncan et al., 1982; Zumbusch et al., 1999), described in detail elsewhere in this book, can provide high-resolution label-free images at video rates without bleaching but has limited chemical resolving power. Usually signal is observed from one or a small number of strong vibrational peaks such as the CH-stretch region of the spectrum (2840-3000 cm−1). us, while it is a very desirable method for

Coherent Raman Scattering Microscopy. Edited by Ji-Xin Cheng and X. Sunney Xie © 2013 CRC Press/ Taylor & Francis Group, LLC. ISBN: 978-1-4398-6765-5.