ABSTRACT
I. Introduction ..................................................................................................35 A. Chemistry .........................................................................................36 B. Polyclonals vs. monoclonals..........................................................36 C. Conjugation of antibodies..............................................................36
II. Production of monoclonal antibodies ......................................................37 A. Continuously proliferating cell lines ...........................................38 B. Human-human hybridomas .........................................................38 C. Large-scale production ...................................................................38
III. Drug-monoclonal antibody conjugates for drug targeting...................39 A. Principles ..........................................................................................39 B. Drug antibody bonding .................................................................39 C. In vitro and in vivo testing .............................................................40
IV. Recent studies with monoclonal antibodies............................................43 A. Highlights of current research ......................................................43
V. Conclusion and basis for future trends....................................................52 References...............................................................................................................54
I. Introduction At the beginning of this century, Paul Ehrlich reported the discovery of antibodies.1 Since that time, many investigators have done extensive work using a wide variety of antibody molecules in immunocytochemistry, radioimmunoassay, and clinical medicine. In 1976, Kohler and Milstein employed a method of somatic-cell hybridization in order to successfully generate a
