ABSTRACT
On average, about one-third of the human genome is actively transcribed. However, the identities of the transcribed genes differ in various cell types and at distinct stages of differentiation and development [1]. The quantitation of gene expression at the cellular level is of central importance for understanding cellular biology and its role in human disease. Recent advances in DNA sequencing, and a variety of other techniques to examine the differential expression of mRNA levels in different cell and tissue types, together with advances in microarray technologies, have contributed to the identification of novel genes and their roles in cellular physiology and disease processes [2-4].
