ABSTRACT
Positive and negative contrast confocal imaging of mitochondria in hepatocytes. Rat hepatocytes were loaded with 1
m
M calcein acetoximethylester at 37
C for 15 min, followed by 250 nM tetramethylrhodamine methylester (TMRM). Cytosolic esterases release and trap green-fluorescing calcein in the cytosol and nucleus (left panel). Dark voids in the green fluorescence image correspond mostly to mitochondria, which exclude calcein. TMRM is a red-fluorescing mitochondrial marker. Each TMRM-labeled mitochondrion in the red fluorescence image (right panel) corresponds to a dark void in the calcein image (left panel). Courtesy of Dr. Ting Qian and John J. Lemasters. (See color figures following page 42.)
FIGURE 6
Mitochondria stained with DASPMI viewed by image intensifier. The image quality and resolution is somewhat less than obtained in microphotographs. The image is transferred from the intensifier to a videocassette. Further image deterioration occurs in the frame grabber SNAPPY, in the attached personal computer and printer.
