ABSTRACT
To localize the skin lesional site and identify inflammatory cells in the skin, skin biopsies are taken at the lesional site and fixed in 10%-buffered formalin for at least 24 hours. Five-
m
m-thick sections are cut and stained with hematoxylin and eosin and examined under a light microscope [12]. Cell-specific immunostaining and histochemical staining can also be used to confirm the identity of different inflammatory cells. For example, the Toluidine-blue staining method is specific for mast cells [14].
